Vitamin B Coenzyme Forms
Updated Mar. 04th, 2026 | Read Time: 4 Minutes | What You Will Learn:
- What Are Coenzyme B Vitamins?
- Benefits of The Coenzyme Form of B Vitamins
- Vitamin B1: Thiamine, TPP and Benfotiamine
- Vitamin B2: Riboflavin, R5P, and FMN/FAD
- Vitamin B3: Niacin, Niacinamide, IHN, NADH
- Vitamin B5: Pantothenic Acid, Pantothenate, and Pantethine
- Vitamin B6: Pyridoxine, pyridoxal, pyridoxamine, and P5P/PLP
- Nutritional Information
- Reader Comments (4)
What Are Coenzyme B Vitamins?
B vitamins are a large group of molecules involved as cofactors in many of our cellular metabolic processes, particularly cellular respiration, which involves the cellular metabolism of fat, protein and carbohydrates into usable energy forms. That means that in order for our bodies to properly live, grow, heal, and stay healthy, we require B vitamins.
Interestingly, each subset of B vitamin comes in a number of different forms, each of which is absorbed and used differently by our bodies. Research has suggested that ingesting some vitamins in their active coenzyme form can help to promote more efficient use in our bodies. In some cases, supplementation with the active coenzyme form of a vitamin, or a combination formula, has been shown to have completely different effects than supplementation with the vitamin alone.
Benefits of The Coenzyme Form of B Vitamins
Each B vitamin is unique in its function and in the difference between the plain vitamin and its active coenzyme form. Please see below to learn more about each.
Vitamin B1: Thiamine, TPP and Benfotiamine
Thiamine is a water-soluble vitamin used in many cellular reactions throughout the body. It is an antioxidant that is associated with brain health, blood cell production, improved cardiovascular, and digestive function.
Thiamine is absorbed from the digestive tract using transport molecules. These molecules can only absorb a limited amount of thiamine at one time. Once inside the body, thiamine is converted to its active form: thiamine pyrophosphate (TPP).
Interestingly, there is a substance called benfotiamine that is a fat-soluble thiamine precursor that can bypass these absorption transport pathways in the gut. Because of this, its absorption is less limited than that of thiamine alone. Although it is not technically thiamine, once absorbed, benfotiamine is also quickly converted into the active coenzyme TPP. In addition to its conversion to active thiamine, benfotiamine has protective effects against the damage caused by glycosylation from excess blood sugar levels in diabetics. Because of this, it can help to prevent diabetes-associated neuropathy and cardiovascular disease.
Vitamin B2: Riboflavin, R5P, and FMN/FAD
Riboflavin is a water-soluble B vitamin that is phosphorylated in the intestines prior to absorption. The phosphorylated riboflavin comes in two different forms: flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), both of which are cofactors in the energy production pathways of our cells.
Riboflavin is required for cellular reproduction and activity; thus, our bodies require this nutrient for growth and repair. Because phosphorylated riboflavin is the form actively absorbed in the intestine, supplementing with it is theorized to improve absorption.
The phosphorylated form of riboflavin found in supplements is often FMN, which is riboflavin-5'-phosphate, and is also called R5P. It is likely that people with proper enzyme function in their guts would not require pre-phosphorylated riboflavin and could instead take it plain.
Vitamin B3: Niacin, Niacinamide, IHN, NADH
Niacin is another water-soluble B vitamin that can be considered non-essential, as the body can produce its own using tryptophan and vitamins B1, B2 and B6. Niacin can be taken in its plain form (as nicotinic acid), or can be taken as/articles/supplements/vitamin-b-3-niacinamide/ or /articles/supplements/vitamin-b-3-ihn/. Each of these forms can be converted into NADH, a major energy production cofactor in the body.
Plain niacin causes the blood vessels in the body to dilate, which causes a "flush" response in the skin. The dilatory response of plain niacin has cardiovascular and anti-allergy benefits. Niacinamide and IHN do not cause this response, and are thus called "no-flush" or "flush-free" niacin. These forms of niacin do not have the same cardiovascular, lipid modifying, or anti-allergy effects, although they do still have the positive effects on energy metabolism and mental health.
Vitamin B5: Pantothenic Acid, Pantothenate, and Pantethine
Pantothenic acid is an essential nutrient that is commonly known as the "anti-stress" vitamin. It is required in the body for the synthesis of coenzyme A (CoA), an active side-group that is used for a wide variety of functions throughout the body. It has a role in nerve signaling, enzyme activation and deactivation, hormone production, and mood neurotransmitter production. In addition, B5 increases the rate of wound healing and promotes blood and immune cell formation.
It can be used topically to treat skin conditions and internally for rheumatoid arthritis. Most commonly, vitamin B5 is known for its stress-adaptive functions. Interestingly, pantethine is called activated, or coenzyme vitamin B, although it does not actually act as pantothenic acid in the body. Instead, pantethine has unique lipid-lowering and cholesterol-balancing properties, and is dosed differently from other forms of this vitamin.
Vitamin B6: Pyridoxine, Pyridoxal, Pyridoxamine, and P5P/PLP
Pyridoxine is probably the most widely used B vitamin in the body. It can be taken in a number of different forms, although it is most commonly found as the precursor form pyridoxal, or as /articles/supplements/vitamin-b-6-2/ (P5P / PLP), which is the active coenzyme form. Because P5P is cleaved back to pyridoxal in the intestines prior to absorption, most people can benefit equally from supplementation of pyridoxal or P5P. Those with an enzymatic deficiency in their ability to re-phosphorylate pyridoxal will benefit from P5P supplementation directly.
The active coenzyme form of this water-soluble vitamin plays a role in many chemical processes, such as cardiovascular health, neurotransmitter production, cellular growth, DNA replication, antibody formation, mood regulation, and it can help prevent "morning sickness" during pregnancy.




Vitamin B supplementation is great for everyone, not just vegan/vegetarians.
Hello Jenni,
Indeed, while vegans and vegetarians may need an extra boost, especially with b-12. However, most people don't get enough b vitamins from their diet alone. If you'd like to learn more about b vitamins, check out our other articles too:
https://www.nationalnutrition.ca/articles/supplements/supplement-articles-vitamin-b-12/
Have a healthy day!
Thank you for another great Vitamin B article. This is a great overview/summary of all the different B vitamin forms. I agree with both you and Jenni that vitamin B supplementation is a great idea for everyone (as opposed to just vegans and vegetarians, which is one common misconception). You do an excellent job demonstrating the important biological function of B vitamins from this article and I hope that other readers have the same takeaway message!: thanks for making chemistry clear and the importance of B-vitamins obvious :)
Hello Rachel,
Indeed, the b family of vitamins are an important part of everyday health and vitamins and minerals are getting harder to get from diet alone due to our soil not being as nutrient dense as it once was. Coenzyme formulas are more bioavailable formulas, allowing you to absorb more of your supplement. Keeping the delicate balance of all the b vitamins is important (unless you have a deficiency in one specifically, of course) so taking a b complex as outlined in this article is beneficial for most people. You can find quality coenzyme b complex formulas here, https://www.nationalnutrition.ca/vitamin-b/b-complex/co-enzyme-b-complex.html
Keep healthy & well